16 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 7.5)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE
Alternate Names for Recombinant Human Calmodulin Protein
CALM
CALM1
CALM2
CALM3
CALML2
calmodulin 1 (phosphorylase kinase, delta)
Calmodulin 1
calmodulin
CaM
CAM1
CAM2
CAM3
CAMB
CAMC
CAMI
CAMIII
CPVT4
DD132
PHKDCAM
phosphorylase kinase, delta subunit
Background
Calmodulin has been known to act as an intracellular calcium sensor protein. When the intracellular Ca2+ concentration increases, calmodulin can bind up to four Ca2+, changing its conformation and regulating cellular functions such as activation or inhibition of a large number of enzymes, ion channels, and receptors. Recombinant human Calmodulin was expressed in E.coli and purified by conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
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FAQs for Calmodulin Recombinant Protein (NBC1-18401). (Showing 1 - 3 of 3 FAQ).
I am looking for an antibody that can bind an engineered calmodulin domain of a fluorescent biosensor. Can you provide me the amino acid sequence that your calmodulin antibodies were raised against so that I can compare them to the sequence I am working with? Can you please provide your sequence identity for this fragment: D Q L T E E Q I A E F K E A F S L F D K D G D G T I T T K E L G T V M R S L G Q N P T E A E L Q D M I N E V D A D G D G T I D F P E F L T M M A R K M W G T D S E E E I R E A F R V F D K D G N G Y I G A A E L R H V M A N L G E R L T D E E V D E M I R V A D I N G D G Q V S Y E E F V Q M M T A K
Please see this link to our available calmodulin antibodies. This sequences shares 94% similarity with human calmodulin and does not share 100% similarity with any known calmodulin sequence. Any of our calmodulin antibodies will be predicted to react with this sequence. Any of our polyclonal antibodies will be a good choice and chances are most of the monoclonal antibodies will cross-react as well although, unfortunately, there is no way to predict this with the monoclonals. NBP1-61548 would be my best recommendation for your assay and sequence.
There is a faint band above the cam band. What is that?
Calmodulin has several post-translational modifications ( UniProt P62158). These are not always present on all proteins in an overexpressed samples. This is the upper band in the sample.
In the SDS-page, is Calmodulin binding calcium or not?
In an SDS-PAGE, the protein is denatured. Calmodulin is not binding calcium in this gel.
Additional Calmodulin Products
Array NBC1-18401
Calmodulin Antibodies
Calmodulin ELISA Kits
Calmodulin Lysate
Research Areas for Calmodulin Recombinant Protein (NBC1-18401)
Find related products by research area.
Cell Cycle and Replication
Signal Transduction
Blogs on Calmodulin
There are no specific blogs for Calmodulin, but you can read our latest blog posts.
![SDS-Page: Recombinant Human Calmodulin Protein [NBC1-18401] - Calmodulin 2, 16kDa (149aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE](http://images.novusbio.com/images/Recombinant-Human-Calmodulin-Protein-SDS-Page-NBC1-18401-img0002.jpg "SDS-Page: Recombinant Human Calmodulin Protein [NBC1-18401] - Calmodulin 2, 16kDa (149aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE")